【关键词】 猪水肿病; 腹泻; F18菌毛; 单克隆抗体; 免疫荧光试验; 黏附试验; 免疫胶体金试验;

【英文关键词】 edema disease; diarrhea; F18 fimbriae; monoclonal antibody; adherence test; immunofluorenscene test; Gold immunolabeling test;

【中文摘要】 仔猪水肿病(porcine edema disease,ED)和断奶仔猪腹泻(postweaning diarrhea,PWD)是由表达F18菌毛的大肠杆菌引起的仔猪常见的两种传染病,在养猪业的生产中危害很大。细菌利用自身的菌毛介导其在肠道内的定居、繁殖,多数产生F18ab菌毛的菌株,同时产生志贺毒素II型变异体(Shiga Toxin Type II Variant,STx2e或SLT-IIv)引起肠毒血症,导致内皮细胞以及小血管和毛细血管的平滑肌坏死,改变血管通透性,进而仔猪出现水肿,当脑部水肿压迫神经组织后,出现神经症状;产F18ac菌株常常产生不耐热肠毒素(heat-liable enterotoxin,LT)或耐热肠毒素(heat-stable enterotoxin,ST),也有些菌株带有SLT-IIv基因,当其菌毛黏附素与小肠上皮细胞的微绒毛和细胞表面的受体相结合,并牢固地黏附于肠黏膜后,细菌定居、繁殖,产生并释放大量肠毒素,对其靶细胞产生毒性作用,肠内水盐代谢平衡失调导致腹泻。 F18菌毛蛋白结构主要有六个部分:A、B、C、D、E、F。其中A亚单位是菌毛的主要结构亚单位,构成菌...

【英文摘要】 Edema disease and diarrhea of weaned piglets that have important impact on stock raising,which are caused by Escherichia coli F18. bacteria invade and propagate in the microvilli of small intestine by fimbriae. Fimbriae expressing F18ab can make exterotoxins with Shiga Toxin Type II Variant ,which can make the death of leiomyoma of endodermis cells and small blood vessel and micro blood vessel ,change thoroughtfare of blood vessel, make oedema of disease pigs. The pigs can show innervation symptom when...