【关键词】 甲砜霉素; HPLC; 药物动力学; 残留; 鸡;

【英文关键词】 Thiamphenicol; HPLC; Pharmacokinetics; Residue; chicken;

【中文摘要】 本试验首次对甲砜霉素在鸡体内药物动力学进行了研究,并建立了甲砜霉素在鸡组织中残留的HPLC检测方法。 选用20只健康杂交肉鸡,体重范围1.75±0.04 kg,随机分成2组,雌雄各半,进行静脉注射和口服给药的药动学研究。静脉注射给药剂量为15 mg/kg b.w,口服给药剂量为30 mg/kg b.w。给药前后不同时间分别从鸡翅下静脉采集血样。用反相高效液相色谱法测定血浆中甲砜霉素的浓度。采用3p97药动学程序软件处理药-时数据。 健康鸡单剂量静注给药后,血药浓度-时间数据符合无吸收二室开放模型,其主要动力学参数分别为:V(c) 0.92±0.01 L/kg,t1/2α0.27±0.02 h,t 1/2β3.46±0.74 h,AUC 11.67±0.57 (mg/l)·h,CL(s) 1.29 L/kg/h。单剂量口服给药血药浓度-时间数据符合一级吸收一室开放模型,其主要动力学参数分别为:Lagtime 0.04±0.01 h,t1/2ka 0.76±0.11 h,t1/2ke 2.16±0.58 h,T(peak) 1.73±0.11 h,C(max) 6.03±0.92μg/m...

【英文摘要】 The pharmacokinetics of thiamphenicol(TAP) were studied following single intravenous (i.v.) (15 mg/kg b.w.) and oral (30 mg/kg b.w.) administration of the drug in healthy chickens. Twenty birds with body weights of 1.71 to 1.79 kg were randomly divided into 2 equal groups of 10 birds each. Blood samples were collected at different interval (0.083~24h) after administration of TAP. TAP in plasma was extracted and de-proteined by ethyl acetate. The concentrations in plasma were determined by high performa...