【关键词】 减毒鼠伤寒沙门氏菌; DNA疫苗; 质粒稳定性; 禽流感; 免疫效力; CpG; DNA;

【英文关键词】 attenuated Salmonella typhimurium; DNA vaccine; plasmid stability; avian influenza virus; immune efficacy; CpG DNA;

【中文摘要】 高致病性禽流感(HPAI)是危害养禽业的烈性传染病之一。虽然常规灭活苗对控制禽流感的发生流行起到了非常重要的作用,但也存在难以克服的缺点,如存在散毒的潜在危险、不能有效激发细胞和黏膜免疫应答等,因此需要研究一种安全、高效的新型疫苗克服上述不足。 减毒沙门氏菌可作为疫苗载体,通过自然黏膜感染途径运送DNA疫苗至宿主特定的免疫器官和细胞,表达外源抗原,激发机体产生保护性的细胞、体液和黏膜免疫应答。减毒沙门氏菌作为载体运送禽流感DNA疫苗,为禽流感的防治带来新思路和新手段。 外源高拷贝质粒在沙门氏菌中通常不稳定,特别是含bla基因的高拷贝质粒在沙门氏菌中不稳定,已成为重组沙门氏菌疫苗载体研究的阻碍之一。另外,CpG DNA作为一种免疫佐剂,可增强DNA疫苗的免疫效果。本研究对高拷贝质粒pcDNA3.1+进行改造,获得了稳定性高的新质粒pmcDNA3.1+,在此基础上,构建出运送禽流感病毒DNA疫苗的减毒鼠伤寒沙门氏菌,并对其免疫生物学特性作了探讨。 1.真核表达质粒pmcDNA3.1+的构建与鉴定 通过除去真核表达质粒pcDNA3.1+中的氨苄青霉素抗性基因(bla基因)的...

【英文摘要】 Highly pathogenic avian influenza (HPAI), a highly contagious disease with worldwide distribution, can cause severe economic losses to the poultry industry with its high percent of disease and death. The killed vaccine has been verified that they are important for controlling the infection and epidemic of this disease, but several disadvantages are unable to be avoided, e.g. potential dangerous of virus distribution and unable to induce cellular and mucosal immune responses. Thus, developing a safe and...