【关键词】 小尾寒羊; 促性腺激素释放激素受体（GnRHR）基因; 骨形态发生蛋白15（BMP15）基因; 骨形态发生蛋白受体IB; （BMPR-IB）基因; PCR-SSCP; PCR-RFLP;

【英文关键词】 Small Tail Han sheep; Gonadotropin releasing hormone receptor（GnRHR） gene; Bone morphogenetic protein 15（BMP15）gene; Bone morphogenetic protein receptor-IB （BMPR-IB） gene; PCR-SSCP; PCR-RFLP;

【中文摘要】 本研究以200头山东省嘉祥种羊场小尾寒羊母羊和30头北京市顺义区杨镇种羊场多赛特羊为研究素材,选用绵羊促性腺激素释放激素受体(gonadotropin releasing hormone receptor, GnRHR)基因、骨形态发生蛋白15 ( bone morphogenetic protein 15, BMP15)基因、骨形态发生蛋白受体IB (bone morphogenetic protein IB receptor, BMPR-IB)基因多态性为研究内容,采用PCR-SSCP技术分析了GnRHR基因外显子2和外显子1部分序列在小尾寒羊、多赛特羊2个绵羊品种中的多态性,采用PCR-RFLP技术检测BMPR-IB基因和BMP15基因在高繁殖力绵羊品种小尾寒羊中的单核苷酸多态性,同时研究这两个基因对小尾寒羊高繁殖力的协同效应,旨在探索三个候选基因与小尾寒羊高繁殖力的关系。得到如下结论:

【英文摘要】 Small Tail Han Sheep (200) comes from ShangDong provice and Dorset sheep(30) comes from BeiJing are studied as experimental material, Polymorphisms of Gonadotropin releasing hormone receptor(GnRHR) gene, Bone morphogenetic protein 15(BMP15)gene and Bone morphogenetic protein receptor (BMPR-IB) gene are detected, The polymorphism of exon 2 and part sequence of exon 1 of GnRHR gene in Small Tail Han sheep and Dorset sheep is analyzed by PCR-SSCP. Single nucleotide polymorphism of BMPR-IB and BMP15 genes ...