【中文摘要】 目的：观察中药提取液在体内外能否有效地抑制R质粒在S．flexneri D15 R-plasmid Cm~R和E．coli 1485 Rif~R之间的接合传递作用。 方法：1．六十种单味中药经乙醇提取制成提取液，分别测定各味中药提取液Sflexneri D15 R-plasmid Cm~R和E．coli 1485 Rif~R的最小抑菌浓度(MIC)，取两菌共同的亚最小抑菌浓度为体外实验浓度。2．体内外R质粒接合传递抑制实验：①体外筛选有效抑制R质粒接合传递的药物：中药提取液与S．flexneri D15 R-plasmid Cm~R和E．coli 1485 Rif~R共同培养24和48小时，并设立七个对照组；计算各味中药和各对照组的R质粒转移率和有效药的R质粒转移抑制率：R质粒转移率(％)=转移接合子数／受体菌菌落数×100％；R质粒转移抑制率(％)=(1—药物作用后的转移接合子数／无药物作用的转移接合子数)×100％。②有效药物体内R质粒接合传递抑制实验：2倍的有效药物体外实验浓度与S．flexneri D15R-plasmid Cm~R和E．coli 1485 Rif~R注入昆明小白鼠腹腔...

【英文摘要】 Objective: To examine a possible influence of the extrat from edible alcohol of Chinese medicinal materials on the R plasmid transfer by conjugation from S.flexneri D15 R-plasmid Cm~R to E.coil 1485 Rif in vitro and in vivo. Methods:1. Make the extrat of 60 Chinese medicinal materials from edible alcohol seperately, and be sure their experimental concentration in vitro:determine the minimal inhibitory concentration(MIC)of the every kind of Chinese medicinal materials of S.flexneri D15 R-plasmid Cm~R and...